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KMID : 0903519990420030262
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1999 Volume.42 No. 3 p.262 ~ p.266
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Isolation , Identification , and Activity of Rosmarinic Acid , a Potent Antioxidant Extracted from Korean Agastache rugosa
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김종범/Kim, Jong Bum
황영수/김정봉/박노동/조강진/Hwang, Young Soo/Kim, Jung Bong/Park, Ro Dong/Cho, Kang Jin
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Abstract
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Thirty plants were screened for their antioxidative activity. The extracts of Agastache rugosa, Schizonepeta tenuifolia and Lycopus lucidus had high free radical(2,2-diphenyl-l-picrylhydrazyl) scavenging activities. Methanol extract of Agastache rugosa was fractionated with hexane, chloroform, ethyl acetate, and water. The ethyl acetate fraction showed the highest antioxidant activity in the DPPH test. The ethyl acetate fraction was applied to Sephadex LH-20 column, and the fractions showing antioxidative activity were collected and used for identification of the substance. The purified substance was applied to mass, IR, UV and NMR spectroscopy. The spectra of mass, IR, UV and NMR implied that the substance is a rosmarinic acids as a kind of phenolic compound. The rosmarinic acid has more antioxidative effect than those of BHT and α-tocopherol in the Rancimat test.
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